Journal: Frontiers in Microbiology

Article Title: Relationship of electrochemical performance and biofilm development of Desulfuromonas acetexigens and Geobacter sulfurreducens in microbial electrolysis cells

doi: 10.3389/fmicb.2026.1753230

Figure Lengend Snippet: Mean measured parameters of current density and biofilm morphology in the duplicate cultivations Des2 A B ¯ , Des3 A B ¯ , and Des5 A B ¯ of D. acetexigens and Geo1 A B ¯ of G. sulfurreducens at an applied potential of 0 mV vs. SHE. The mean values of current density (J), average biovolume ( ( B V ¯ A B C ) , and mean biofilm thickness ( L ¯ F ) are shown. The displayed cultivation time corresponds to the shorter experiment of the duplicate. (a) Mean values of duplicate Des2 A B ¯ , inoulated at OD 600 = 0.15. (b) Mean values of duplicate Des3 A B ¯ , inoculated at OD 600 = 0.35. (c) Mean values of duplicate Des5 A B ¯ , inoculated with 1 mL cryostock culture. (d) Mean values of duplicate Des5 A B ¯ , inoculated with different cell densities (Geo1A: OD 600 = 0.15; Geo1B: OD 600 = 0.35).

Article Snippet: Desulfuromonas acetexigens 2873 (DSM 1397) and Geobacter sulfurreducens PCA (DSM 12127) were both activated as lyophilized cultures as described by the German Collection of Microorganisms and Cell Cultures (DSMZ, Germany).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Relationship of electrochemical performance and biofilm development of Desulfuromonas acetexigens and Geobacter sulfurreducens in microbial electrolysis cells

doi: 10.3389/fmicb.2026.1753230

Figure Lengend Snippet: Taxonomic classification of the 16S amplicon sequencing of D. acetexigens cultivations Des3B and Geobacter sulfurreducens cultivation Geo1B at species level. Samples were taken from the cryostocks used for inoculation, the anodic and cathodic biofilms, and the planktonic cell fraction of the microbial flow cells. A representation of the taxonomically classified bacterial classes is provided in the . (a) Desulfuromonas acetexigens cultivation Des3B. (b) Geobacter sulfurreducens cultivation Geo1A.

Article Snippet: Desulfuromonas acetexigens 2873 (DSM 1397) and Geobacter sulfurreducens PCA (DSM 12127) were both activated as lyophilized cultures as described by the German Collection of Microorganisms and Cell Cultures (DSMZ, Germany).

Techniques: Amplification, Sequencing

Journal: Bio-protocol

Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants

doi: 10.21769/BioProtoc.5614

Figure Lengend Snippet: . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells (DU145), in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.

Article Snippet: Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.

Techniques: Activation Assay, Control, Derivative Assay, Expressing